TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63]
概述
产品名称
TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TIE2 aa 1-769.
种属反应性
Human
验证应用
WB, FC
分子量
Predicted band size: 126 kDa
阳性对照
EA.hy926 cell lysate, EA.hy926.
偶联
unconjugated
克隆号
PSH06-63
反应性数据
| WB | FC | |
|---|---|---|
| Human |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
FC
-
1:1,000
靶点
功能
Angiopoietin-1 receptor also known as CD202B (cluster of differentiation 202B) is a protein that in humans is encoded by the TEK gene. Also known as TIE2, it is an angiopoietin receptor. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. (TEK is closely related to the TIE receptor tyrosine kinase). This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. TEK has also been suggested as a marker for nucleus pulposus progenitor cells, from the intervertebral disc, which upon activation by Angiopoietin-1 starts to multiply and differentiate. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. In cancer patients, TEK (Tie2) is expressed in a subpopulation of monocytes that home in on the tumor and are essential for the formation of new blood vessels there.
背景文献
1. Du S et al. Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancer. Cancer Lett. 2022 Mar
2. Duran CL et al. Targeting Tie2 in the Tumor Microenvironment: From Angiogenesis to Dissemination. Cancers (Basel). 2021 Nov
亚细胞定位
Cell membrane, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Secreted.
UNIPROT
别名
Angiopoietin 1 receptor antibody
Angiopoietin-1 receptor antibody
CD202b antibody
CD202b antigen antibody
Endothelial tyrosine kinase antibody
Endothelium specific receptor tyrosine kinase 2 antibody
hTIE 2 antibody
hTIE2 antibody
Hyk antibody
p140 TEK antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of TIE2 on different lysates with Rabbit anti-TIE2 antibody (HA722732) at 1/1,000 dilution.
Lane 1: EA.hy926 cell lysate
Lane 2: HEK-293 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 126 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722732) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Flow cytometric analysis of HEK-293 (left, negative) and EA.hy926 (right, positive) cells labeling TIE2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722732, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Endothelial GNAQ p.R183Q mutation confers hemoporfin-mediated photodynamic therapy resistance and drives pathological angiogenesis via the angiopoietin-2/TIE2/PI3K/AKT pathway
期刊: Frontiers In Cell And Developmental Biology
DOI: 10.3389/fcell.2025.1622961
IF: 4.3
应用: WB
反应种属: Human
发表时间: 2025 Aug
同靶点 & 同通路的产品
TIE2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
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