Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]
Catalog# HA721351
Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]
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WB
-
IF-Cell
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Human
概述
产品名称
Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Poliovirus Receptor aa 194-343 / 417.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 45 kDa
阳性对照
HUVEC cell lysate, U87-MG cell lysate, A549 cell lysates, HUVEC.
偶联
unconjugated
克隆号
JE45-53
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:1,000-1:2,000
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IF-Cell
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1:100
靶点
功能
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication.
背景文献
1. Wu B et al. Poliovirus receptor (PVR)-like protein cosignaling network: new opportunities for cancer immunotherapy. J Exp Clin Cancer Res. 2021 Aug
2. Li Y et al. Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury. Liver Int. 2022 Dec
亚细胞定位
Cell membrane, Secreted.
UNIPROT
别名
CD155 antibody
CD155 antigen antibody
FLJ25946 antibody
HVED antibody
mE4 antibody
NECL 5 antibody
Necl-5 antibody
Necl5 antibody
Nectin like 5 antibody
Nectin like protein 5 antibody
展开图片
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Western blot analysis of Poliovirus Receptor on different lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: U87-MG cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 75 kDa
Exposure time: 4 minutes 39 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Poliovirus Receptor on A549 cell lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HUVEC cells labeling Poliovirus Receptor with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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