Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
Catalog# HA722526
Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
-
WB
-
Human
概述
产品名称
Phospho-Paxillin (Y118) Recombinant Rabbit Monoclonal Antibody [JE09-10]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human Paxillin.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 65 kDa
阳性对照
A431 cell lysate, PANC-1 cell lysate.
偶联
unconjugated
克隆号
JE09-10
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Paxillin is a protein that in humans is encoded by the PXN gene. Paxillin is expressed at focal adhesions of non-striated cells and at costameres of striated muscle cells, and it functions to adhere cells to the extracellular matrix. Mutations in PXN as well as abnormal expression of paxillin protein has been implicated in the progression of various cancers.
背景文献
1. Ripamonti M et al. Paxillin: A Hub for Mechano-Transduction from the beta3 Integrin-Talin-Kindlin Axis. Front Cell Dev Biol. 2022 Apr
2. Zhang Y et al. Direct active Fyn-paxillin interaction regulates vascular smooth muscle cell migration. J Smooth Muscle Res. 2023
亚细胞定位
Cytoplasm, cytoskeleton, Cell junction, focal adhesion, Cytoplasm, cell cortex.
UNIPROT
别名
FLJ16691 antibody
FLJ23042 antibody
Paired box protein Pax 1 antibody
PAX 1 antibody
PAX1 antibody
PAXI_HUMAN antibody
Paxillin alpha antibody
Paxillin antibody
PXN antibody
PXN protein antibody
图片
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Western blot analysis of Phospho-Paxillin (Y118) on different lysates with Rabbit anti-Phospho-Paxillin (Y118) antibody (HA722526) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: PANC-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722526) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-Paxillin (Y118) on different lysates with Rabbit anti-Phospho-Paxillin (Y118) antibody (HA722526) at 1/1,000 dilution and pan Paxillin antibody (ET1607-22) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 10 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722526) at 1/1,000 dilution and pan Paxillin antibody (ET1607-22) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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