PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]
Catalog# HA601588
PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]
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WB
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]
抗体类型
Recombinant Mouse Monoclonal Antibody
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 63 kDa
阳性对照
Mouse heart tissue lysate, Mouse liver tissue lysate, Rat placenta tissue lysate, Rat heart tissue lysate, HeLa treated with 10μM CCCP for 24 hours cell lysate.
偶联
unconjugated
克隆号
PSH22-50
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
靶点
功能
A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons.
背景文献
1. Quinn PMJ et al. PINK1/PARKIN signalling in neurodegeneration and neuroinflammation. Acta Neuropathol Commun. 2020 Nov
2. Han R et al. PINK1-PRKN mediated mitophagy: differences between in vitro and in vivo models. Autophagy. 2023 May
亚细胞定位
SwissProt: Q9BXM7 Human SwissProt: Q99MQ3 Mouse SwissProt: B5DFG1 Rat
别名
BRPK antibody
FLJ27236 antibody
mitochondrial antibody
PARK 6 antibody
PARK6 antibody
Phosphatase and Tensin Homolog antibody
PINK 1 antibody
PINK1 antibody
PINK1_HUMAN antibody
Protein kinase BRPK antibody
展开图片
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Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat placenta tissue lysate
Lane 4: Rat heart tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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