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PINK1 Rabbit Polyclonal Antibody

RMB: 900 特惠 1500

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Catalog# ER1706-27

PINK1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

PINK1 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within Human PINK1 aa 94-581 / 581.

种属反应性

Human

验证应用

WB

分子量

Predicted band size: 63 kDa

阳性对照

HeLa treated with 10μM CCCP for 24 hours cell lysate.

偶联

unconjugated

RRID

AB_3069043

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

靶点

功能

A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons.

背景文献

暂无

序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

组织特异性

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

翻译后修饰

Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.; Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import. In depolarized mitochondria, cleaved by OMA1 that acts as a backup protease, promoting its subsequent degradation by the proteasome.

亚细胞定位

Mitochondrion outer membrane, Mitochondrion inner membrane, Cytoplasm, cytosol.

UNIPROT

SwissProt: Q9BXM7 Human

别名

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

PARK6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

PINK1 antibody

PINK1_HUMAN antibody

Protein kinase BRPK antibody

展开

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (<a href="/products/ER1706-27" style="font-weight: bold;text-decoration: underline;">ER1706-27</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1706-27" style="font-weight: bold;text-decoration: underline;">ER1706-27</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (ER1706-27) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. IF
  4. IF-cell

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Conjugate: unconjugated

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metafield image

PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]

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Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

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