PERP Recombinant Rabbit Monoclonal Antibody [JU04-51]
Rmb: 880 特惠 1500
Catalog# ET7106-59
PERP Recombinant Rabbit Monoclonal Antibody [JU04-51]
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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unconjugated
概述
产品名称
PERP Recombinant Rabbit Monoclonal Antibody [JU04-51]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human PERP aa 144-193 / 193.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 21 kDa
阳性对照
A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HaCaT (Human immortalized keratinocyte) cell lysate, rat skin tissue lysate, human skin tissue, mouse small intestine tissue.
偶联
unconjugated
克隆号
JU04-51
RRID
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | |
|---|---|
| human |
|
| rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
-
1:50-1:200
靶点
功能
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
背景文献
1. Bonkobara M et al. Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes. Br J Dermatol 148:654-664 (2003).
2. Otsuki T et al. Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res 12:117-126 (2005).
序列相似性
Belongs to the TMEM47 family.
组织特异性
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
亚细胞定位
Cell junction. Membrane.
别名
1110017A08Rik an tibody dJ496H19.1 antibody
KCP 1 antibody
KCP-1 antibody
KCP1 antibody
Keratinocyte associated protein 1 antibody
Keratinocyte-associated protein 1 antibody
Keratinocytes associated protein 1 antibody
KRTCAP 1 antibody
KRTCAP1 antibody
p53 apoptosis effector related to PMP 22 antibody
展开图片
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Western blot analysis of PERP on different lysates with Rabbit anti-PERP antibody (ET7106-59) at 1/5,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lane 2: HaCaT (Human immortalized keratinocyte) cell lysate
Lane 3: Rat skin tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7106-59, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-PERP antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PERP antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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