PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]
概述
产品名称
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human PERK aa 51-450 / 1,116.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 125 kDa
阳性对照
MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate.
偶联
unconjugated
克隆号
PSH0-81
RRID
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Patients with mutations in this gene develop Wolcott-Rallison syndrome. EIF2AK3 has been shown to interact with DNAJC3, NFE2L2, and endoplasmic reticulum chaperone BiP (Hsp70).
背景文献
1. Zhang D et al. A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis. Nat Cell Biol. 2022 May
2. Raines LN et al. PERK is a critical metabolic hub for immunosuppressive function in macrophages. Nat Immunol. 2022 Mar
亚细胞定位
Endoplasmic reticulum membrane.
UNIPROT
别名
DKFZp781H1925 antibody
E2AK3_HUMAN antibody
EC 2.7.11.1 antibody
Eif2ak3 antibody
Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
Heme regulated EIF2 alpha kinase antibody
HRI antibody
HsPEK antibody
Pancreatic eIF2 alpha kinase antibody
展开图片
-
Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA721510) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: U-87 MG cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (16 µg/Lane)
Predicted band size: 125 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response
期刊: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI: 10.1016/j.ecoenv.2025.119006
IF: 6.1
应用: WB
反应种属: Human
发表时间: 2025 Sept
-
Ethanol-induced liver injury is alleviated by chrysin via increasing MRP2 transporter expression and enhancing the Nrf2-mediated adaptive response
期刊: Naunyn-Schmiedebergs Archives Of Pharmacology
DOI: 10.1007/s00210-025-04774-9
IF: 3.1
应用: WB
反应种属: Rat
发表时间: 2025 Oct
-
Sappanchalcone Suppresses NSCLC by Oxidative Stress-Driven DNA Damage and ER Stress Activation through PIEZO1 Modulation
期刊: iScience
DOI: 10.1016/j.isci.2025.114057
IF: 4.1
应用: WB
反应种属: Human
发表时间: 2025 Nov
-
Endoplasmic Reticulum-Targeted Photodynamic Therapy Combined with Autophagy Inhibition to Enhance Synergistic Immunotherapy for Triple-Negative Breast Cancer
期刊: Chemistry - A European Journal
DOI: 10.1002/chem.202500146
IF: 3.9
应用:
反应种属:
发表时间: 2025 May
-
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
期刊: Research Square
DOI: 10.21203/rs.3.rs-4950334/v1
IF:
应用: WB
反应种属: Mouse
发表时间: 2024 Oct
同靶点 & 同通路的产品
PERK Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
浙公网安备 33019202000643号