PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free
Catalog# HA750895
PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free
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WB
-
IHC-P
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FC
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Human
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Mouse
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Rat
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HA722020
含抗保成分
-
unconjugated
概述
产品名称
PDE10A Recombinant Rabbit Monoclonal Antibody [PSH03-61] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human PDE10A aa 400-779 / 779.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 88 kDa
阳性对照
Human brain tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, SH-SY5Y.
偶联
unconjugated
克隆号
PSH03-61
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:20,000
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FC
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1:1,000
靶点
功能
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.
背景文献
1. Chen S et al. PDE10A Inactivation Prevents Doxorubicin-Induced Cardiotoxicity and Tumor Growth. Circ Res. 2023 Jul
2. Menniti FS et al. PDE10A Inhibitors-Clinical Failure or Window Into Antipsychotic Drug Action? Front Neurosci. 2021 Jan
亚细胞定位
Cytoplasm, cytosol.
别名
5''-cyclic phosphodiesterase 10A antibody
cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
cAMP and cAMP-inhibited cGMP 3'' antibody
FLJ11894 antibody
HSPDE 10A antibody
HSPDE10A antibody
PDE 10A antibody
PDE10_HUMAN antibody
Pde10a antibody
Phosphodiesterase 10A antibody
展开图片
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Western blot analysis of PDE10A on different lysates with Rabbit anti-PDE10A antibody (HA750895) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750895) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE10A antibody (HA750895) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750895) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PDE10A antibody (HA750895) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750895) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of SH-SY5Y cells labeling PDE10A.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750895, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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