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iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]

RMB: 3280

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Catalog# HA720167F

iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]

Application

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human NeuN aa 20-60.

种属反应性

Human, Mouse, Rat

验证应用

IF-Tissue, IHC-Fr

分子量

Predicted band size: 34 kDa

阳性对照

Mouse brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue.

偶联

iFluor™ 594

克隆号

SR45-07

RRID

AB_3072104

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IF-Tissue

  • 1:50

  • IHC-Fr

  • 1:100

靶点

功能

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

背景文献

1. Patel TP et al. Single-neuron NMDA receptor phenotype influences neuronal rewiring and reintegration following traumatic injury. J Neurosci 34:4200-13 (2014).

2. Kaur P et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014).

亚细胞定位

Nucleus, Cytoplasm.

UNIPROT #

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene: 143966 Rat

别名

FLJ56884 antibody

FLJ58356 antibody

Fox-1 homolog C antibody

fox1 homolog C antibody

Fox3 antibody

FOX3NeuN antibody

hexaribonucleotide binding protein 3 antibody

HRNBP3 antibody

NEUN antibody

neuronal nuclei antibody

展开

图片

  • Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>, iFluor&trade; 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (HA720167F).

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded mouse cerebral cortex tissue labeling NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>, iFluor&trade; 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded mouse cerebral cortex tissue labeling NeuN (HA720167F).

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (<a href="/products/HA720167F" style="font-weight: bold;text-decoration: underline;">HA720167F</a>, iFluor&trade; 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling NeuN (HA720167F).

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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