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NMDAR2B Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# HA500208

NMDAR2B Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

预测的种属支持售后

概述

产品名称

NMDAR2B Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within human NMDAR2B aa 1100-1300 / 1484.

种属反应性

Mouse, Rat (Predicted: Human)

预测的种属支持售后

验证应用

WB, IF-Cell, IHC-P, FC

分子量

predicated 166 kDa

阳性对照

Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, A549, rat cerebellum tissue, MG-63.

偶联

unconjugated

RRID

AB_3071305

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:100-1:500

靶点

功能

This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

背景文献

1. Fajrin FA. et. al. Ginger extract and its compound, 6-shogaol, attenuates painful diabetic neuropathy in mice via reducing TRPV1 and NMDAR2B expressions in the spinal cord. J Ethnopharmacol. 2020 Mar

2. Ferreira DG. et. al. alpha-synuclein interacts with PrP(C) to induce cognitive impairment through mGluR5 and NMDAR2B. Nat Neurosci. 2017 Nov

亚细胞定位

Late endosome, cytoskeleton, cell membrane, postsynaptic cell membrane, lysosome.

UNIPROT #

SwissProt: Q13224 Human

SwissProt: Q01097 Mouse

SwissProt: Q00960 Rat

别名

AW490526 antibody

EIEE27 antibody

Glutamate [NMDA] receptor subunit epsilon 2 antibody

Glutamate [NMDA] receptor subunit epsilon-2 antibody

Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody

Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody

Glutamate receptor ionotropic NMDA2B antibody

Glutamate receptor subunit epsilon 2 antibody

Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody

GRIN 2B antibody

展开

图片

  • Western blot analysis of NMDAR2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500208" style="font-weight: bold;text-decoration: underline;">HA500208</a>, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br />Lane 3: Mouse hippocampus tissue lysate

    Western blot analysis of NMDAR2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500208, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Mouse hippocampus tissue lysate

  • ICC staining of NMDAR2B in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500208" style="font-weight: bold;text-decoration: underline;">HA500208</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of NMDAR2B in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500208, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NMDAR2B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500208" style="font-weight: bold;text-decoration: underline;">HA500208</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NMDAR2B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500208, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of NMDAR2B was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA500208" style="font-weight: bold;text-decoration: underline;">HA500208</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NMDAR2B was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500208, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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