Mouse/Rat MMP-3 Recombinant Rabbit Monoclonal Antibody [PSH23-35] - BSA and Azide free (Detector)
Rmb: 5600
概述
产品名称
Mouse/Rat MMP-3 Recombinant Rabbit Monoclonal Antibody [PSH23-35] - BSA and Azide free (Detector)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse MMP-3 aa 18-477 (HA211280).
种属反应性
Mouse, Rat
验证应用
ELISA(Det)
阳性对照
Recombinant Mouse MMP-3 protein (HA211280).
偶联
unconjugated
克隆号
PSH23-35
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| Elisa | |
|---|---|
| mouse |
|
| rat |
|
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-34] to Mouse/Rat MMP-3 (Capture) (HA724365) and Recombinant Mouse MMP-3 protein (HA211280) as the standard. The reference range value is 19.5-5,000 pg/mL.
靶点
功能
Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.
背景文献
1. Seehawer M et al. Loss of Kmt2c or Kmt2d drives brain metastasis via KDM6A-dependent upregulation of MMP3. Nat Cell Biol. 2024 Jul
2. Jiang J et al. MMP3 at the crossroads: Linking molecular pathways to disease diagnosis and therapy. Pharmacol Res. 2025 Jun
亚细胞定位
Secreted, extracellular space, extracellular matrix.
别名
CHDS6 antibody
Matrix metalloproteinase 3 antibody
Matrix metalloproteinase-3 antibody
MGC126102 antibody
MGC126103 antibody
MGC126104 antibody
MMP 3 antibody
MMP-3 antibody
MMP3 antibody
Proteoglycanase antibody
展开图片
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![Sandwich ELISA analysis of Mouse MMP-3 matched pair antibodies<br /><br />Capture: <a href="/products/HA724365" style="font-weight: bold;text-decoration: underline;">HA724365</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]<br />Detector: <a href="/products/HA724366" style="font-weight: bold;text-decoration: underline;">HA724366</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724365" style="font-weight: bold;text-decoration: underline;">HA724365</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-3 protein (<a href="/products/HA211280" style="font-weight: bold;text-decoration: underline;">HA211280</a>) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA724366" style="font-weight: bold;text-decoration: underline;">HA724366</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724366_1.png?v=20260908001034)
Sandwich ELISA analysis of Mouse MMP-3 matched pair antibodies
Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]
Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724365) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-3 protein (HA211280) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724366, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native MMP3 in Mouse and Rat serum.<br /><br />Capture: <a href="/products/HA724365" style="font-weight: bold;text-decoration: underline;">HA724365</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]<br />Detector: <a href="/products/HA724366" style="font-weight: bold;text-decoration: underline;">HA724366</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]<br /><br />Interpolated concentration of native MMP3 was measured in duplicate at different sample concentrations and interpolated from the MMP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 74,092 pg/ml in Mouse serum and 11,892 pg/ml in Rat serum.](https://storage.huabio.cn/huabio/productImg/HA724366_2.png?v=20260908001034)
Interpolated concentrations of native MMP3 in Mouse and Rat serum.
Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]
Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]
Interpolated concentration of native MMP3 was measured in duplicate at different sample concentrations and interpolated from the MMP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 74,092 pg/ml in Mouse serum and 11,892 pg/ml in Rat serum. -
![Interpolated concentrations of spiked MMP3 in cell culture media samples.<br /><br />Capture: <a href="/products/HA724365" style="font-weight: bold;text-decoration: underline;">HA724365</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]<br />Detector: <a href="/products/HA724366" style="font-weight: bold;text-decoration: underline;">HA724366</a>, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]<br /><br />The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA724366_3.png?v=20260908001034)
Interpolated concentrations of spiked MMP3 in cell culture media samples.
Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34]
Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35]
The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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