Mouse IL-27 P28/IL-30 DTSet enzyme-linked immunoassay kit
Catalog# DSEM005105；DSEM005115
Mouse IL-27 P28/IL-30 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
概述
产品名称
Mouse IL-27 P28/IL-30 DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0282 | 0.0291 | 0.0287 | |
| 31.25 | 0.2052 | 0.1973 | 0.2013 | 0.1726 |
| 62.50 | 0.4267 | 0.3899 | 0.4083 | 0.3797 |
| 125.00 | 0.7059 | 0.7679 | 0.7369 | 0.7083 |
| 250.00 | 1.3142 | 1.2542 | 1.2842 | 1.2556 |
| 500.00 | 2.2904 | 2.1274 | 2.2089 | 2.1803 |
| 1000.00 | 3.1309 | 3.4612 | 3.2961 | 3.2674 |
| 2000.00 | 4.1228 | 4.0689 | 4.0959 | 4.0672 |
实验类型
夹心酶联免疫吸附测定（Sandwich Elisa）
种属反应性
Mouse
检测方法
比色法
实验检测时长
120min
标准曲线范围
31.25 pg/ml -2000 pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
加样体积
10 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 （Streptavidin-HRP）
所需的其他材料
DTSet辅助试剂盒（5板）：包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液（PBS）、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买：
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
UNIPROT
功能
IL-27是一种异二聚体细胞激素，由EBI3（与IL-12和IL-23的p40亚基相关）和p28（与IL-12的p35链相关）组成。IL-27由单核细胞、内皮细胞和树突状细胞表达，通过由IL-27 R α/WSX-1/TCCR和gp130组成的受体复合物传递信号。IL-27诱导IL-12受体在未发育的CD4 T细胞上表达，使其对后续IL-12诱导的Th1发育产生产生敏感。它还通过限制T和NKT细胞活性，发挥抗炎细胞因子的作用。
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