Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]

功能

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.

背景文献

1. Ishida K et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014).

2. Beck J et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013).

序列相似性

Belongs to the bacterial solute-binding protein 1 family.

UNIPROT #

SwissProt: P0AEX9 EscherichiaColi

SwissProt: P0AEY0 EscherichiaColi

别名

ECK4026 antibody

JW3994 antibody

Mal E antibody

MalE antibody

malJ antibody

Maltodextrin binding protein antibody

Maltose ABC transporter periplasmic protein antibody

Maltose binding periplasmic protein antibody

MMBP antibody

Periplasmic maltose binding protein antibody