Fibrinogen Mouse Monoclonal Antibody [D5-B3]

功能

The plasma glycoprotein Fibrinogen is synthesized in the liver and comprises three structurally different subunits: a, b and g. Fibrinogen is important in platelet aggregation, the final step of the coagulation cascade (i.e. the formation of Fibrin) and determination of plasma viscosity and erythrocyte aggregation. It is both constitutively expressed and inducible during an acute phase reaction. Hemostasis following tissue injury deploys essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Following vascular injury, Fibrinogen is cleaved by Thrombin to form Fibrin, which is the most abundant component of blood clots. The cleavage products of Fibrinogen regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types.

背景文献

1. Spraggon G et al. Crystal structure of a recombinant alphaEC domain from human fibrinogen-420. Proc Natl Acad Sci USA 95:9099-9104 (1998).

2. Kollman JM et al. Crystal structure of human fibrinogen. Biochemistry 48:3877-3886 (2009).

组织特异性

Detected in blood plasma (at protein level).

翻译后修饰

The alpha chain is normally not N-glycosylated. It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studies (PubMed:16335952 and PubMed:19159218), but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency.; O-glycosylated.; Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.; About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.; Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.; Phosphorylated by FAM20C in the extracellular medium.

亚细胞定位

Secreted.

UNIPROT

SwissProt: P02671 Human

SwissProt: P02675 Human

SwissProt: P02679 Human

别名