CD80 Mouse Monoclonal Antibody [8-E5]

Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (M1007-10) at 1/1,000 dilution.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 60 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin- embedded human brain tissue using using anti-CD80 Mouse mAb (Cat. # M1007-10).

☑ Relative expression (RE)

Flow cytometric analysis of HUVEC (left, negative) and Raji (right, positive) cells labeling CD80.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (M1007-10, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).