Lck Rabbit Polyclonal Antibody
Catalog# HA500280
Lck Rabbit Polyclonal Antibody
-
WB
-
FC
-
Human
-
Rat
-
unconjugated
概述
产品名称
Lck Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human Lck aa 100-300 / 509.
种属反应性
Human, Rat
验证应用
WB, FC
分子量
58 kDa
阳性对照
Rat thymus tissue lysate, Jurkat cell lysate, Hela.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
FC
-
1:500-1:1,000
靶点
功能
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
背景文献
1. Bozso SJ. et. al. The role of competing mechanisms on Lck regulation. Immunol Res. 2020 Oct
2. Bommhardt U. et. al. Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy. Int J Mol Sci. 2019 Jul
亚细胞定位
Cytosol, Cell membrane.
别名
IMD22 antibody
LCK antibody
Lck p56 antibody
LCK proto-oncogene, Src family tyrosine kinase antibody
LCK_HUMAN antibody
Leukocyte C-terminal Src kinase antibody
LSK antibody
Lymphocyte cell specific protein tyrosine kinase antibody
Lymphocyte cell-specific protein-tyrosine kinase antibody
Lymphocyte specific protein tyrosine kinase antibody
展开图片
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Western blot analysis of Lck on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500280, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat thymus tissue lysate
Lane 2: Jurkat cell lysate -
Flow cytometric analysis of Lck was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500280, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
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Application: WB,IF-Cell,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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