LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91]
Catalog# HA724336
LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91]
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WB
-
IF-Cell
-
FC
-
IP
-
Human
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Mouse
-
Rat
-
Monkey
-
Pig
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unconjugated
概述
产品名称
LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human LDHB aa 1-334.
种属反应性
Human, Mouse, Rat, Monkey, Pig
验证应用
WB, IF-Cell, FC, IP
分子量
Predicted band size: 37 kDa
阳性对照
HEK-293 (Human embryonic kidney cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, LNCaP (Human prostate cancer cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate, Rat kidney tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.
偶联
unconjugated
克隆号
PSH22-91
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:20,000-1:50,000
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IF-Cell
-
1:500
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FC
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1:1,000
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IP
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1-2μg/sample
靶点
功能
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.
背景文献
1. Fan S et al. LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection. Autophagy. 2021 Sep
2. Hong W et al. PGC-1alpha loss promotes mitochondrial protein lactylation in acetaminophen-induced liver injury via the LDHB-lactate axis. Pharmacol Res. 2024 Jul
亚细胞定位
Cytoplasm, Mitochondrion inner membrane.
别名
Epididymis secretory protein Li 281 antibody
HEL S 281 antibody
L lactate dehydrogenase B chain antibody
L-lactate dehydrogenase B chain antibody
Lactate dehydrogenase H chain antibody
LDH B antibody
LDH H antibody
LDH heart subunit antibody
LDH-B antibody
LDH-H antibody
展开图片
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Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/20,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cells) cell lysate
Lane 2: Huh7 (Human liver cancer cells) cell lysate
Lane 3: LNCaP (Human prostate cancer cells) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 21 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724336, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/20,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724336, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/50,000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Rat kidney tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Pig brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724336, 1/50,000 in 5% NFDM/TBST, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724336, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (M120505-2, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Application: Flow Cytometry (Intra)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724336 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Immunoprecipitation (IP)
LDHB was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724336 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724336 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA724336 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724336 in HeLa cell lysate
Exposure time: 2 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724336, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
LDHB Recombinant Rabbit Monoclonal Antibody [SU39-06] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,IP
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
LDHB Recombinant Rabbit Monoclonal Antibody [SU39-06]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,IP
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
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