Iba1 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS068
Iba1 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
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unconjugated
概述
产品名称
Iba1 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within N-terminal human Iba1.
种属反应性
Mouse
验证应用
mIHC
分子量
Predicted band size: 17 kDa
阳性对照
Mouse brain tissue.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
背景文献
1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).
2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).
组织特异性
Detected in T-lymphocytes and peripheral blood mononuclear cells.
翻译后修饰
Phosphorylated on serine residues.
亚细胞定位
Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.
UNIPROT
别名
AIF 1 antibody
AIF-1 antibody
Aif1 antibody
AIF1 protein antibody
AIF1_HUMAN antibody
Allograft inflammatory factor 1 antibody
Allograft inflammatory factor 1 splice variant G antibody
allograft inflammatory factor-1 splice variant Hara-1 antibody
balloon angioplasty responsive transcription antibody
BART 1 antibody
展开图片
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with PAX6 (IRS066), NeuN, Iba1 (IRS068) and MAP2 (IRS071) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Iba1 antibody (IRS068) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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