F4/80 Recombinant Rabbit Monoclonal Antibody
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Catalog# IRS052
F4/80 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
概述
产品名称
F4/80 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse F4/80 aa 1-650 / 931.
种属反应性
Mouse
验证应用
mIHC
分子量
Predicted band size: 102 kDa
阳性对照
Mouse spleen tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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mIHC
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1:100
靶点
功能
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
背景文献
1. Deng R et al. Periosteal CD68+ F4/80+ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1. Adv Sci (Weinh). 2022 Jan
2. Shin AE et al. F4/80+Ly6Chigh Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology. 2023 Apr
亚细胞定位
Cell membrane.
UNIPROT
别名
ADGRE1 antibody
Adhesion G protein coupled receptor E1 antibody
Adhesion G protein-coupled receptor E1 antibody
AGRE1_HUMAN antibody
Cell surface glycoprotein EMR1 antibody
Cell surface glycoprotein F4/80 antibody
DD7A5 7 antibody
Egf like module containing mucin like hormone receptor like 1 antibody
Egf like module containing mucin like hormone receptor like sequence 1 antibody
EGF like module receptor 1 antibody
展开图片
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (IRS052) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), Ki67 (IRS057), CD8 and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, F4/80 (IRS052), CD4 (IRS051), CD206 (IRS049) and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1 (IRS059), F4/80 (IRS052), FOXP3 (IRS053), CD11c and CD4 (IRS051) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), B220, CD206 (IRS049), CD4 (IRS051) and CD8 antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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