IL-4R Recombinant Rabbit Monoclonal Antibody [PSH10-86]
Catalog# HA723253
IL-4R Recombinant Rabbit Monoclonal Antibody [PSH10-86]
-
WB
-
IP
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
HA751378
不含抗保成分
-
unconjugated
概述
产品名称
IL-4R Recombinant Rabbit Monoclonal Antibody [PSH10-86]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human IL-4R aa 1-232.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP, IF-Cell
分子量
Predicted band size: 90 kDa
阳性对照
THP-1 cell lysate, Daudi cell lysate, 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, Ramos cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Mouse pancreas tissue lysate, PC-12.
偶联
unconjugated
克隆号
PSH10-86
反应性数据
| WB | IP | IF-Cell | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
-
IF-Cell
-
1:100
靶点
功能
The interleukin 4 receptor is a type I cytokine receptor. It is a heterodimer, that is, composed of two subunits. IL4R is the human gene coding for IL-4Rα, the subunit which combines with either common gamma chain (γc, forming the type I IL4 receptor) or with IL-13Rα1 (forming the type II IL4 receptor). This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. Among T cells, the encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitis, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. Interactions of IL-4 with TNFα promote structural changes to vascular endothelial cells, thus playing an important role in tissue inflammation.
背景文献
1. Aranda CJ et al. IgG memory B cells expressing IL4R and FCER2 are associated with atopic diseases. Allergy. 2023 Mar
亚细胞定位
Cell membrane; Secreted.
别名
CD124 antibody
IL 4R alpha antibody
IL-4 receptor subunit alpha antibody
IL-4-binding protein antibody
IL-4R subunit alpha antibody
IL-4R-alpha antibody
IL-4RA antibody
IL4-BP antibody
Il4r antibody
IL4R nirs variant 1 antibody
展开图片
-
Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA723253) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: Daudi cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: Ramos cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse spleen tissue lysate (40 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)
Predicted band size: 90 kDa
Observed band size: 110 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
IL-4R was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723253 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723253 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA723253 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA723253 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 51 seconds; ECL: K1801 -
Immunocytochemistry analysis of PC-12 cells labeling IL-4R with Rabbit anti-IL-4R antibody (HA723253) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4R antibody (HA723253) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Biotin Conjugated Human IL-4R Recombinant Rabbit Monoclonal Antibody [PSH11-46] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
Human IL-4R Recombinant Rabbit Monoclonal Antibody [PSH11-45] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
IL-4R Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
IL-4R Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IL-4R Rabbit Polyclonal Antibody
Application: WB,IF-Cell
Reactivity: Human
Conjugate: unconjugated
IL-4R Recombinant Rabbit Monoclonal Antibody [PSH10-86] - BSA and Azide free
Application: WB,IP,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
浙公网安备 33019202000643号