HRP Conjugated Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-16] - Detector
概述
产品名称
HRP Conjugated Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-16] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human ICAM1 aa 28-480 (HA210857).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human ICAM1 protein (HA210857).
偶联
HRP
克隆号
PSH10-16
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-15] to Human ICAM1 antibody (Capture) (HA723184) and Recombinant Human ICAM1 protein (HA210857) as the standard.
-
ELISA
-
Use at an assay dependent concentration.
靶点
功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. Acts as a receptor for Coxsackievirus A21 capsid proteins. Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Interacts (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells.
背景文献
1. Hayashi T., Takahashi T., Motoya S., Ishida T., Itoh F., Adachi M., Hinoda Y., Imai K. MUC1 mucin core protein binds to the domain 1 of ICAM-1. Digestion 63 Suppl. 1:87-92 (2001)
2. van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., Burridge K. RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. J. Cell Biol. 178:1279-1293 (2007)
亚细胞定位
Membrane.
UNIPROT
别名
Antigen identified by monoclonal antibody BB2 antibody
BB 2 antibody
BB2 antibody
CD 54 antibody
CD_antigen=CD54 antibody
CD54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
ICAM-1 antibody
展开图片
-
Sandwich ELISA analysis of Human ICAM1 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723184) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ICAM1 protein (HA210857) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723474H, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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