Human Apolipoprotein E Recombinant Rabbit Monoclonal Antibody [PSH20-20] - BSA and Azide free (Capture)

Sandwich ELISA analysis of Human Apolipoprotein E matched pair antibodies

Capture: HA724131, Human Apolipoprotein E Rabbit mAb [PSH20-18]
Detector: HA724130, Human Apolipoprotein E Rabbit mAb [PSH20-19]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted APOE protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724130, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Apolipoprotein E in Human serum.

Capture: HA724131, Human Apolipoprotein E Rabbit mAb [PSH20-18]
Detector: HA724130, Human Apolipoprotein E Rabbit mAb [PSH20-19]

Interpolated concentration of native Apolipoprotein E was measured in duplicate at different sample concentrations and interpolated from the Apolipoprotein E standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Apolipoprotein E concentration was determined to be 12.5 ug/ml in Human serum.

Interpolated concentrations of spiked Apolipoprotein E in cell culture media samples.

Capture: HA724131, Human Apolipoprotein E Rabbit mAb [PSH20-18]
Detector: HA724130, Human Apolipoprotein E Rabbit mAb [PSH20-19]

The concentrations of Apolipoprotein E were measured in duplicates, interpolated from the Apolipoprotein E standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).