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Anti-DYKDDDDK (Flag) Magnetic Beads

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Catalog# HAK21011

Anti-DYKDDDDK (Flag) Magnetic Beads

This product has been cited in peer reviewed publications, see list HERE

概述

产品描述

产品包装:

 

产品编号

产品名称

产品形式

包装规格(V/V, 1/5)

HAK21011-1 mL

Anti-DYKDDDDK Magnetic Beads

20% 磁珠悬液

1 mL

HAK21011-5 mL

Anti-DYKDDDDK Magnetic Beads

20% 磁珠悬液

5 mL

HAK21011-25 mL

Anti-DYKDDDDK Magnetic Beads

20% 磁珠悬液

25 mL

产品描述:

Anti-DYKDDDDK 免疫磁珠来源于 Anti-DYKDDDDK [A2-A4]重组鼠单克隆抗体,与直径为 30-150 μm 的琼脂糖磁珠共价偶联。 [A2-A4]抗体能识别包含 Flag 多肽序列(DYKDDDDK)的融合蛋白。[A2-A4]可特异性地结合哺乳动物和细菌提取物中的甲硫氨酸修饰的 N 端 Flag 融合蛋白、C 端 Flag 融合蛋白。

Anti-DYKDDDDK 免疫磁珠可用于常用的免疫沉淀程序检测和捕获 Flag 标记的融合蛋白。可以通过磁性吸附实现快速高效的分离,无需离心操作,以促进实验过程。例如重复洗涤或处理多孔板中的多个样品。

产品特性

产品性能

产品形式:Anti-DYKDDDDK 免疫磁珠保存在 PBS,pH7.4 添加防腐剂的缓冲液中,以 20%的悬浮液形式提供。

结合能力:每 1mL 纯磁珠可结合≥0.6mg Flag 融合蛋白,测试蛋白C 端融合 Flag 标签,分子量为 70KDa 的蛋白。

表 1 磁珠结合能力

 

纯磁珠的体积(ul)

结合能力(ug)

20

〜12

50

〜30

100

〜60

200

〜120

注:不要使用磁力搅拌系统。磁力搅拌系统会破坏磁珠结构。

辅助设备:磁力架、离心管、96 孔板。

存放说明

Anti-DYKDDDDK免疫磁珠采用蓝冰运输。建议在2-8℃下储存,可稳定保存2年。请勿-20℃保存,冷冻磁珠将不可逆转地损坏磁珠结构。

图片

  • Immunoprecipitation<br />Anti-DYKDDDDK [A2-A4] Magnetic Beads: Cat.No. HAK21011: Purification of Two Different FLAG-tagged fusion protein from HeLa transfected with Flag-fusion protein expression vector. 10ul Anti-FLAG [A2-A4] Magnetic Beads was used for IP per lane.<br />Lane 1, 2, 3, 4: Hela transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate<br />Lane 1’, 2’, 3’, 4’: Hela transfected with Flag-fusion protein expression vector containing an C-terminal Flag tag, whole cell lysate

    Immunoprecipitation
    Anti-DYKDDDDK [A2-A4] Magnetic Beads: Cat.No. HAK21011: Purification of Two Different FLAG-tagged fusion protein from HeLa transfected with Flag-fusion protein expression vector. 10ul Anti-FLAG [A2-A4] Magnetic Beads was used for IP per lane.
    Lane 1, 2, 3, 4: Hela transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate
    Lane 1’, 2’, 3’, 4’: Hela transfected with Flag-fusion protein expression vector containing an C-terminal Flag tag, whole cell lysate

  • Immunoprecipitation<br />Lane 1, 2, 3, 4, 5: Input, 5μL, 10μL, 15μL, 20 μL Anti-DYKDDDDK [A2-A4] Magnetic Beads suspension were add to the same volume of the Hela lysate transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate.

    Immunoprecipitation
    Lane 1, 2, 3, 4, 5: Input, 5μL, 10μL, 15μL, 20 μL Anti-DYKDDDDK [A2-A4] Magnetic Beads suspension were add to the same volume of the Hela lysate transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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