NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
Rmb: 5600
Catalog# HA751854
NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
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WB
-
IHC-P
-
IHC-Fr
-
IF-Tissue
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Human
-
Mouse
-
Rat
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ET1703-57
含抗保成分
-
Cynomolgus monkey
预测的种属支持售后
-
unconjugated
概述
产品名称
NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human NEFM aa 651-692 / 916.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey)
预测的种属支持售后
验证应用
WB, IHC-P, IHC-Fr, IF-Tissue
靶点分子量
Predicted band size: 102 kDa
阳性对照
Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, 293T cell lysate, Rat brain tissue lysate, Mouse brain tissue lysate.
偶联
unconjugated
克隆号
JM11-20
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:2,000
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IHC-Fr
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1:500
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IF-Tissue
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1:500
靶点
功能
Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.
背景文献
1. Li D et al. NEFM DNA methylation correlates with immune infiltration and survival in breast cancer. Clin Epigenetics. 2021 May
2. Sohrabi N et al. Regulatory Role of Insulin on Endogenous L1 ORF1 and NEFM Gene Expression through PI3K Signaling Pathway Specifically in Neuroblastoma Cell Line. Iran J Public Health. 2023 Mar
序列相似性
Belongs to the intermediate filament family.
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.; Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
亚细胞定位
Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.
别名
150kDa medium antibody
NEF3 antibody
NEFM antibody
Neurofilament 3 antibody
NF160 antibody
NFM antibody
图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-M antibody (HA751854) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751854) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-M antibody (HA751854) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751854) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-M antibody (HA751854) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751854) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of NF-M on different lysates with Rabbit anti-NF-M antibody (HA751854) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)
Lane 3: Rat liver tissue lysate (negative) (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751854) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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