Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54] - BSA and Azide free
Rmb: 5600
Catalog# HA751567
Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54] - BSA and Azide free
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WB
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IHC-P
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Mouse
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Rat
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HA723760
含抗保成分
-
unconjugated
概述
产品名称
Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human RYR-2 aa 1,301-1,600.
种属反应性
Mouse, Rat
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 565 kDa
阳性对照
Mouse heart tissue lysate, Rat heart tissue lysate, mouse heart tissue.
偶联
unconjugated
克隆号
PSH15-54
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Ryanodine receptor 2 (RYR2) is one of a class of ryanodine receptors and a protein found primarily in cardiac muscle. In humans, it is encoded by the RYR2 gene. In the process of cardiac calcium-induced calcium release, RYR2 is the major mediator for sarcoplasmic release of stored calcium ions. The RYR2 protein functions as the major component of a calcium channel located in the sarcoplasmic reticulum that supplies ions to the cardiac muscle during systole. To enable cardiac muscle contraction, calcium influx through voltage-gated L-type calcium channels in the plasma membrane allows calcium ions to bind to RYR2 located on the sarcoplasmic reticulum. This binding causes the release of calcium through RYR2 from the sarcoplasmic reticulum into the cytosol, where it binds to the C domain of troponin, which shifts tropomyosin and allows the myosin ATPase to bind to actin, enabling cardiac muscle contraction. RYR2 channels are associated with many cellular functions, including mitochondrial metabolism, gene expression and cell survival, in addition to their role in cardiomyocyte contraction.
背景文献
1. Steinberg C et al. RYR2-ryanodinopathies: from calcium overload to calcium deficiency. Europace. 2023 Jun
2. Fowler ED et al. Molecular, Subcellular, and Arrhythmogenic Mechanisms in Genetic RyR2 Disease. Biomolecules. 2022 Jul
亚细胞定位
Sarcoplasmic reticulum membrane.
别名
ARVC2 antibody
ARVD2 antibody
Cardiac muscle ryanodine receptor antibody
Cardiac muscle ryanodine receptor-calcium release channel antibody
hRYR-2 antibody
ryanodine receptor 2 (cardiac) antibody
Ryanodine receptor 2 antibody
RyR antibody
RYR-2 antibody
RyR2 antibody
展开图片
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☑ Relative expression (RE)
This data was developed using HA723760, the same antibody clone in a different buffer formulation.
Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/2,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate (negative)
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat heart tissue lysate
Lane 5: Rat skeletal muscle tissue lysate (negative)
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 565 kDa
Observed band size: 565 kDa
Exposure time: 1 minute 7 seconds; ECL: K1802;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723760) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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