p16INK4a Recombinant Rabbit Monoclonal Antibody [PSH15-40] - BSA and Azide free
Rmb: 5600
Catalog# HA751555
p16INK4a Recombinant Rabbit Monoclonal Antibody [PSH15-40] - BSA and Azide free
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WB
-
IHC-P
-
IP
-
Mouse
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HA723746
含抗保成分
-
unconjugated
概述
产品名称
p16INK4a Recombinant Rabbit Monoclonal Antibody [PSH15-40] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
种属反应性
Mouse
验证应用
WB, IHC-P, IP
靶点分子量
Predicted band size: 18 kDa
阳性对照
A20 cell lysate, MEF cell lysate, mouse spleen tissue.
偶联
unconjugated
克隆号
PSH15-40
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:200
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IP
-
1:1,000
靶点
功能
p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. It is encoded by the CDKN2A gene. A deletion (the omission of a part of the DNA sequence during replication) in this gene can result in insufficient or non-functional p16, accelerating the cell cycle and resulting in many types of cancer. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer. p16 was discovered in 1993. It is a protein with 148 amino acids and a molecular weight of 16 kDa that comprises four ankyrin repeats. The name of p16 is derived from its molecular weight, and the alternative name p16INK4a refers to its role in inhibiting cyclin-dependent kinase CDK4.
背景文献
1. Safwan-Zaiter H et al. P16INK4A-More Than a Senescence Marker. Life (Basel). 2022 Aug
2. Shi J et al. P16ink4a overexpression ameliorates cardiac remodeling of mouse following myocardial infarction via CDK4/pRb pathway. Biochem Biophys Res Commun. 2022 Mar
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
CCM2 antibody
CDK4 inhibitor p16 INK4 antibody
CDK4I antibody
CDKN2 antibody
CDKN2A antibody
Cell cycle negative regulator beta antibody
CMM2 antibody
Cyclin dependent kinase 4 inhibitor A antibody
Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
Cyclin Dependent Kinase Inhibitor 2A antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (HA751555) at 1/2,000 dilution.
Lane 1: A20 cell lysate
Lane 2: NIH/3T3 cell lysate (negative)
Lane 3: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751555) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-p16INK4a antibody (HA751555) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
p16INK4a was immunoprecipitated from 0.2 mg MEF cell lysate with HA751555 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751555 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MEF cell lysate (input)
Lane 2: HA751555 IP in MEF cell lysate
Lane 3: Rabbit IgG instead of HA751555 in MEF cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1801
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