Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free
Catalog# HA751530
Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free
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IHC-Fr
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IHC-P
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Mouse
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Rat
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HA723668
含抗保成分
-
unconjugated
概述
产品名称
Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse Homeobox protein cut-like 1 aa 1,000-1,200.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P
分子量
Predicted band size: 166 kDa
阳性对照
Mouse cerebrum tissue, rat cerebrum tissue.
偶联
unconjugated
克隆号
PSH14-70
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:200-1:500
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IHC-P
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1:500-1:1,000
靶点
功能
Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.
背景文献
1. Liu N et al. CUX1, A Controversial Player in Tumor Development. Front Oncol. 2020 May
2. Jotte MRM et al. The significance of CUX1 and chromosome 7 in myeloid malignancies. Curr Opin Hematol. 2022 Mar
亚细胞定位
Nucleus.
别名
CCAAT displacement protein antibody
CDP antibody
CDP/Cut antibody
CDP1 antibody
Clox antibody
COY1 antibody
Cut homeobox antibody
Cut homolog antibody
Cut like 1 antibody
Cut like homeobox 1 antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: cerebrum
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
Application: IHC-Fr
Species: Rat
Site: cerebrum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA751530) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751530) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA751530) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751530) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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