CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51] - BSA and Azide free
Catalog# HA751470
CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51] - BSA and Azide free
-
IHC-Fr
-
IHC-P
-
WB
-
Mouse
-
Rat
-
HA723540
含抗保成分
-
unconjugated
概述
产品名称
CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human CACNA1E aa 1,964-2,313.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P, WB
分子量
Predicted band size: 262 kDa
阳性对照
Mouse striatum tissue, mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH13-51
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-Fr
-
1:500
-
IHC-P
-
1:3,000-1:6,000
-
WB
-
1:5,000
靶点
功能
The R-type calcium channel is a type of voltage-dependent calcium channel. Like the others of this class, the α1 subunit forms the pore through which calcium enters the cell and determines most of the channel's properties. This α1 subunit is also known as the calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E) or Cav2.3 which in humans is encoded by the CACNA1E gene. They are strongly expressed in cortex, hippocampus, striatum, amygdala and interpeduncular nucleus. They are poorly understood, but like Q-type calcium channels, they appear to be present in cerebellar granule cells. They have a high threshold of activation and relatively slow kinetics.
背景文献
1. Schneider T et al. Cav2.3 R-type calcium channels: from its discovery to pathogenic de novo CACNA1E variants: a historical perspective. Pflugers Arch. 2020 Jul
2. Royer-Bertrand B et al. De novo variants in CACNA1E found in patients with intellectual disability, developmental regression and social cognition deficit but no seizures. Mol Autism. 2021 Oct
亚细胞定位
Membrane.
别名
alpha-1 polypeptide antibody
BII antibody
Brain calcium channel II antibody
CAC 1E antibody
CAC1E antibody
CAC1E_HUMAN antibody
CACH 6 antibody
CACH6 antibody
CACNA1E antibody
Cacnl1 a6 antibody
展开图片
-
Application: IHC-Fr
Species: Mouse
Site: hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751470) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/6,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751470) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751470) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/6,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751470) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751470) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody (HA751470) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate (no heat)
Lane 3: Mouse lung tissue lysate (negative) (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 262 kDa
Observed band size: 257 kDa
Exposure time: 46 seconds; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751470) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号