AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
Rmb: 5600
Catalog# HA751467
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
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WB
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IHC-Fr
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IHC-P
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IF-Tissue
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Mouse
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Rat
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HA723537
含抗保成分
-
Human
预测的种属支持售后
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unconjugated
概述
产品名称
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human AMH aa 411-560.
种属反应性
Mouse, Rat (Predicted: Human)
预测的种属支持售后
验证应用
WB, IHC-Fr, IHC-P, IF-Tissue
靶点分子量
Predicted band size: 59 kDa
阳性对照
Mouse ovary tissue lysate, Mouse testis tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.
偶联
unconjugated
克隆号
PSH13-48
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | IHC-Fr | IF-Cell | IF-Tissue | |
|---|---|---|---|---|---|
| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis. In humans, it is encoded by the AMH gene, on chromosome 19p13.3, while its receptor is encoded by the AMHR2 gene on chromosome 12. AMH is activated by SOX9 in the Sertoli cells of the male fetus. Its expression inhibits the development of the female reproductive tract, or Müllerian ducts (paramesonephric ducts), in the male embryo, thereby arresting the development of fallopian tubes, uterus, and upper vagina. AMH expression is critical to sex differentiation at a specific time during fetal development, and appears to be tightly regulated by nuclear receptor SF-1, transcription GATA factors, sex-reversal gene DAX1, and follicle-stimulating hormone (FSH). Mutations in both the AMH gene and the type II AMH receptor have been shown to cause the persistence of Müllerian derivatives in males that are otherwise normally masculinized.
背景文献
1. Cedars MI. Evaluation of Female Fertility-AMH and Ovarian Reserve Testing. J Clin Endocrinol Metab. 2022 May
2. Howard JA et al. Molecular Mechanisms of AMH Signaling. Front Endocrinol (Lausanne). 2022 Jun
亚细胞定位
Secreted.
别名
Anti muellerian hormone antibody
MIF antibody
MIS antibody
Muellerian inhibiting factor antibody
Mullerian inhibiting substance antibody
图片
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☑ Relative expression (RE)
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Western blot analysis of AMH on different lysates with Rabbit anti-AMH antibody (HA723537) at 1/5,000 dilution.
Lane 1: Mouse ovary tissue lysate
Lane 2: Mouse spleen tissue lysate (negative)
Lane 3: Mouse testis tissue lysate
Lane 4: Rat ovary tissue lysate
Lane 5: Rat spleen tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: Lane 1-3: 42 seconds; Lane 4-5: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: ovary
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: ovary
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-AMH antibody (HA723537) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723537) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-AMH antibody (HA723537) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723537) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Application: IF-Tissue
Species: Mouse
Site: ovary
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using HA723537, the same antibody clone in a different buffer formulation.
Application: IF-Tissue
Species: Rat
Site: ovary
Sample: Paraffin-embedded section
Antibody concentration: 1/500
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48]
Application: WB,IHC-Fr,IHC-P,IF-Tissue
Reactivity: Mouse,Rat,Human
Conjugate: unconjugated
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