GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91] - BSA and Azide free
Rmb: 5600
Catalog# HA751027
GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91] - BSA and Azide free
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WB
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IHC-P
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IP
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IHC-Fr
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Human
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Mouse
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Rat
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HA722478
含抗保成分
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IHC-P, IP, IHC-Fr
靶点分子量
Predicted band size: 48 kDa
阳性对照
Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse brain tissue.
偶联
unconjugated
克隆号
PSH05-91
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:1,000
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IP
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1-2μg/sample
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IHC-Fr
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1:500
靶点
功能
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This gene encodes a member of the G protein-coupled inwardly-rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including heart rate in cardiac cells and circuit activity in neuronal cells, through G-protein coupled receptor stimulation. Mutations in this gene are associated with Keppen-Lubinsky Syndrome, a rare condition characterized by severe developmental delay, facial dysmorphism, and intellectual disability.
背景文献
1. Reyes S., Fu Y., Double K., Thompson L., Kirik D., Paxinos G., Halliday G.M. GIRK2 expression in dopamine neurons of the substantia nigra and ventral tegmental area. J Comp Neurol 520:2591-2607 (2012)
2. Cramer N.P., Best T.K., Stoffel M., Siarey R.J., Galdzicki Z. GABAB-GIRK2-mediated signaling in Down syndrome. Adv Pharmacol 58:397-426 (2010)
亚细胞定位
Membrane.
别名
inwardly rectifying subfamily J member 6 antibody
BIR1 antibody
G protein activated inward rectifier potassium channel 2 antibody
G protein-activated inward rectifier potassium channel 2 antibody
GIRK-2 antibody
GIRK2 antibody
hiGIRK2 antibody
Inward rectifier K(+) channel Kir3.2 antibody
Inward rectifier potassium channel KIR3.2 antibody
KATP-2 antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat brain tissue lysate (no heat)
Lane 4: Rat hippocampus tissue lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 35/48 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751027 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751027 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA751027 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA751027 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 26 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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