P2Y12 Recombinant Rabbit Monoclonal Antibody [PSH04-48] - BSA and Azide free
Catalog# HA750957
P2Y12 Recombinant Rabbit Monoclonal Antibody [PSH04-48] - BSA and Azide free
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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HA722133
含抗保成分
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
P2Y12 Recombinant Rabbit Monoclonal Antibody [PSH04-48] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-P, IHC-Fr, IF-Tissue
分子量
Predicted band size: 39 kDa
阳性对照
Human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebrum tissue, mouse hippocampus tissue, rat cerebrum tissue.
偶联
unconjugated
克隆号
PSH04-48
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:1,000
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IHC-Fr
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1:1,000-1:2000
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IF-Tissue
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1:500
靶点
功能
P2Y12 is a chemoreceptor for adenosine diphosphate (ADP) that belongs to the Gi class of a group of G protein-coupled (GPCR) purinergic receptors. This P2Y receptor family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. The P2Y12 receptor is involved in platelet aggregation and is thus a biological target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene. In the field of purinergic signaling, the P2Y12 protein on the periphery is found mainly but not exclusively on the surface of blood platelets, and is an important regulator in blood clotting. In the central nervous system, this receptor has been found expressed exclusively on microglia, where it is necessary for physiological and pathological microglial actions, such as monitoring neuronal functions and microglial neuroprotection.
背景文献
1. Chen J et al. P2Y12 inhibitor clopidogrel inhibits renal fibrosis by blocking macrophage-to-myofibroblast transition. Mol Ther. 2022 Sep
2. Rolling CC et al. P2Y12 Inhibition Suppresses Proinflammatory Platelet-Monocyte Interactions. Thromb Haemost. 2023 Feb
亚细胞定位
Cell membrane.
别名
ADP glucose receptor antibody
ADP-glucose receptor antibody
ADPG R antibody
ADPG-R antibody
ADPGR antibody
BDPLT8 antibody
G protein coupled receptor SP1999 antibody
Gi coupled ADP receptor HORK 3 antibody
Gi coupled ADP receptor HORK3 antibody
HORK 3 antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:2,000
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:2,000
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12 antibody (HA750957) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750957) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-P2Y12 antibody (HA750957) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750957) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-P2Y12 antibody (HA750957) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750957) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"