F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87] - BSA and Azide free
Catalog# HA750761
F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87] - BSA and Azide free
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WB
-
IHC-P
-
IF-Tissue
-
IHC-Fr
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Mouse
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HA721745
含抗保成分
-
unconjugated
概述
产品名称
F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse F4/80 aa 1-650 / 931.
种属反应性
Mouse
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr
分子量
Predicted band size: 102 kDa
阳性对照
RAW264.7 cell lysate, mouse liver tissue, mouse spleen tissue, mouse lung tissue.
偶联
unconjugated
克隆号
PSH01-87
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:5,000
-
IF-Tissue
-
1:500
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IHC-Fr
-
1:500-1:1,000
靶点
功能
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
背景文献
1. Wang J, Wu X, Dai W, et al. The CCDC43-ADRM1 axis regulated by YY1, promotes proliferation and metastasis of gastric cancer. Cancer Lett. 2020 Jul 10; 482:90-101.
2. Fejzo MS, Anderson L, von Euw EM, et al. Amplification Target ADRM1: Role as an Oncogene and Therapeutic Target for Ovarian Cancer. Int J Mol Sci. 2013 Feb 1;14(2):3094-109.
亚细胞定位
Cell membrane.
UNIPROT
别名
ADGRE1 antibody
Adhesion G protein coupled receptor E1 antibody
Adhesion G protein-coupled receptor E1 antibody
AGRE1_HUMAN antibody
Cell surface glycoprotein EMR1 antibody
Cell surface glycoprotein F4/80 antibody
DD7A5 7 antibody
Egf like module containing mucin like hormone receptor like 1 antibody
Egf like module containing mucin like hormone receptor like sequence 1 antibody
EGF like module receptor 1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750761) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 102 kDa
Observed band size: 160 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750761) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IHC-Fr
Species: Mouse
Site: Liver
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Spleen
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Liver
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: IF-tissue
Species: Mouse
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500
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