KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77] - BSA and Azide free
Catalog# HA750751
KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77] - BSA and Azide free
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WB
-
IHC-P
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Human
-
Mouse
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HA721729
含抗保成分
-
unconjugated
概述
产品名称
KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human KDM3A aa 1-400 / 1,321.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 147 kDa
阳性对照
HCT116 cell lysate, Hela cell lysate, MCF7 cell lysate, Ramos cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human testis tissue, mouse testis tissue.
偶联
unconjugated
克隆号
PSH01-77
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
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1:200-1:1,000
靶点
功能
KDM3A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
背景文献
1. Yoo J, Jeon YH, Cho HY, et al. Advances in Histone Demethylase KDM3A as a Cancer Therapeutic Target. Cancers (Basel). 2020 Apr 28;12(5):1098.
2. Qian X, Li X, Shi Z, et al. KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis. Mol Cell. 2019 Dec 19;76(6):885-895.
亚细胞定位
Cytoplasm, Nucleus
别名
DKFZp686A24246 antibody
DKFZp686P07111 antibody
JHDM2A antibody
JHMD2A antibody
JmjC domain-containing histone demethylation protein 2A antibody
JMJD1 antibody
JMJD1A antibody
Jumonji C domain containing histone demethylase 2A antibody
Jumonji domain containing 1 antibody
Jumonji domain containing 1A antibody
展开图片
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Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA750751) at 1/1,000 dilution.
Lane 1: HCT116 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Ramos cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 147 kDa
Observed band size: 150 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750751) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA750751) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750751) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA750751) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750751) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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