CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
Catalog# HA750422
CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
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WB
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Human
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ET1704-97
含抗保成分
-
unconjugated
概述
产品名称
CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human CXCR3 aa 278-298 (Extracellular)/368.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 41 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, PANC-1 cell lysate, K-562 cell lysate, HT-29 cell lysate, Human lung tissue lysate.
偶联
unconjugated
克隆号
JA61-33
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:5,000
靶点
功能
Chemokine receptor CXCR3 is a Gαi protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. CXCR3 is able to regulate leukocyte trafficking. Binding of chemokines to CXCR3 induces various cellular responses, most notably integrin activation, cytoskeletal changes and chemotactic migration. CXCR3-ligand interaction attracts Th1 cells and promotes Th1 cell maturation. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. A hallmark of CXCR3 is its prominent expression in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, CXCL9, CXCL10 and CXCL11 are commonly produced by local cells in inflammatory lesion, suggesting that CXCR3 and its chemokines participate in the recruitment of inflammatory cells. Additionally, CXCR3 has been implicated in wound healing.
背景文献
1. Tanaka A et al. Th2 and regulatory immune reactions contribute to IgG4 production and the initiation of Mikulicz disease. Arthritis Rheum 64:254-63 (2012).
2. Song CH et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011).
序列相似性
Belongs to the G-protein coupled receptor 1 family.
组织特异性
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).
翻译后修饰
Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.; N-glycosylated.
亚细胞定位
Cell membrane.
UNIPROT
别名
C-X-C chemokine receptor type 3 antibody
CD 183 antibody
CD182 antibody
CD183 antibody
Chemokine (C X C motif) receptor 3 antibody
Chemokine (C X C) receptor 3 antibody
Chemokine CXC Motif Receptor 3 antibody
CKR L2 antibody
CKR-L2 antibody
CKRL2 antibody
展开图片
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Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (HA750422) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: K-562 cell lysate
Lane 5: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750422) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (HA750422) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (10 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time: 57 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750422) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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