EAAT1 Recombinant Rabbit Monoclonal Antibody [JA30-35] - BSA and Azide free
Rmb: 5600
Catalog# HA750415
EAAT1 Recombinant Rabbit Monoclonal Antibody [JA30-35] - BSA and Azide free
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WB
-
IHC-P
-
IF-Tissue
-
IHC-Fr
-
Human
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Mouse
-
Rat
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ET1704-54
含抗保成分
-
Cynomolgus monkey
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Pig
预测的种属支持售后
-
unconjugated
概述
产品名称
EAAT1 Recombinant Rabbit Monoclonal Antibody [JA30-35] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human EAAT1 aa 171-220 / 542.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr
靶点分子量
Predicted band size: 60 kDa
阳性对照
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
偶联
unconjugated
克隆号
JA30-35
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
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IHC-P
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1:1,000-1:8,000
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IF-Tissue
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1:500
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IHC-Fr
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1:500
靶点
功能
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.
背景文献
1. Xu NJ et al. Morphine withdrawal increases glutamate uptake and surface expression of glutamate transporter GLT1 at hippocampal synapses. J Neurosci. 23(11): 4775-4784 (2003).
2. Ueda, Hideho et al. Caveolin-1 Localization in Müller Cells of the Retina ACTA HISTOCHEMICA ET CYTOCHEMICA. 35: 423-428 (2002).
序列相似性
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A3 subfamily.
组织特异性
Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
翻译后修饰
Glycosylated.
亚细胞定位
Cell membrane.
别名
GLAST-1
SLC1A3
EAAT1
GLAST
GLAST1
图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Application: IF-tissue
Species: Rat
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-EAAT1 antibody (HA750415) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750415) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EAAT1 antibody (HA750415) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750415) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT1 antibody (HA750415) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750415) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Western blot analysis of EAAT1 on different lysates with Rabbit anti-EAAT1 antibody (HA750415) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse brain tissue lysate treated with deglycosylation
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate (no heat)
Lane 6: Rat cerebellum tissue lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60-250 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750415) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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