AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98] - BSA and Azide free
Catalog# HA750378
AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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ET1703-34
含抗保成分
-
Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human AQP1 aa 245-269 / 269.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IF-Tissue, IHC-P
分子量
Predicted band size: 28 kDa
阳性对照
Human lung tissue lysate, rat kidney tissue lysate, rat lung tissue lysate, mouse kidney tissue lysate, mouse lung tissue lysate, MCF-7, Hela, SW480, human spleen tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
克隆号
JM10-98
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000-1:5,000
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IF-Tissue
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1:500-1:2,000
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IHC-P
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1:5,000
靶点
功能
Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of Aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most Aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP1 is an integral membrane protein expressed in erythrocytes and renal tubule cells.
背景文献
1. Lobo NC et al. Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine. BMC Cancer 16:485 (2016).
2. Méndez-Gómez HR et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol Ther Methods Clin Dev 2:15037 (2015).
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family.
组织特异性
Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
亚细胞定位
Cell membrane.
别名
AQP 1 antibody
AQP CHIP antibody
AQP-1 antibody
AQP1 antibody
AQP1_HUMAN antibody
aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
aquaporin 1 (Colton blood group) antibody
Aquaporin CHIP antibody
Aquaporin-1 antibody
Aquaporin-CHIP antibody
展开图片
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Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/2,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Rat kidney tissue lysate
Lane 3: Rat kidney tissue lysate (no heat)
Lane 4: Rat lung tissue lysate
Lane 5: Rat lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate (no heat)
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"