HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06] - BSA and Azide free
Catalog# HA750258
HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06] - BSA and Azide free
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WB
-
IP
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Species independent
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ET1611-49
含抗保成分
-
unconjugated
概述
产品名称
HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.
种属反应性
Species independent
验证应用
WB, IP
阳性对照
HA tag recombinant protein, C-terminal HA-tagged recombinant protein.
偶联
unconjugated
克隆号
SN07-06
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
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IP
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2-5 µg/ml.
靶点
功能
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA tag recombinant protein.
背景文献
1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).
别名
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
图片
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Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750258, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with HA750258 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/5,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.
Lane 1: HA Tag fusion protein lysate (input).
Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"