HRP Conjugated Human TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH18-79] - Detector
概述
产品名称
HRP Conjugated Human TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH18-79] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TIMP1 aa 24-207 (HA211003).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human TIMP1 protein (HA211003).
偶联
HRP
克隆号
PSH18-79
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-78] to Human TIMP1 antibody (Capture) (HA725367) and Recombinant Human TIMP1 protein (HA211003) as the standard. The reference range value is 3,125-100,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
靶点
功能
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
背景文献
1. Guccini I et al. Senescence Reprogramming by TIMP1 Deficiency Promotes Prostate Cancer Metastasis. Cancer Cell. 2021 Jan
2. Schoeps B et al. TIMP1 Triggers Neutrophil Extracellular Trap Formation in Pancreatic Cancer. Cancer Res. 2021 Jul
亚细胞定位
Secreted.
UNIPROT
别名
Clgi antibody
Collagenase inhibitor antibody
Collagenase inhibitor, Human antibody
EPA antibody
EPO antibody
Erythroid Potentiating Activity antibody
Erythroid-potentiating activity antibody
Fibroblast collagenase inhibitor antibody
FLJ90373 antibody
HCI antibody
展开图片
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![Sandwich ELISA analysis of human TIMP1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725367" style="font-weight: bold;text-decoration: underline;">HA725367</a>, Human TIMP1 Rabbit mAb [PSH18-78]<br />Detector: <a href="/products/HA725368" style="font-weight: bold;text-decoration: underline;">HA725368</a>, Human TIMP1 Rabbit mAb [PSH18-79]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725367" style="font-weight: bold;text-decoration: underline;">HA725367</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TIMP1 protein (<a href="/products/HA211003" style="font-weight: bold;text-decoration: underline;">HA211003</a>) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725368" style="font-weight: bold;text-decoration: underline;">HA725368</a>, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725368H_1.jpg?v=20260401003254)
Sandwich ELISA analysis of human TIMP1 matched pair antibodies
Capture: HA725367, Human TIMP1 Rabbit mAb [PSH18-78]
Detector: HA725368, Human TIMP1 Rabbit mAb [PSH18-79]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725367) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TIMP1 protein (HA211003) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725368, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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