Human beta Synuclein Recombinant Rabbit Monoclonal Antibody [PSH19-25] - BSA and Azide free (Detector)
概述
产品名称
Human beta Synuclein Recombinant Rabbit Monoclonal Antibody [PSH19-25] - BSA and Azide free (Detector)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human beta Synuclein aa 1-134 (HA211157).
种属反应性
Human, Mouse, Rat
验证应用
ELISA(Det)
阳性对照
Recombinant Human beta Synuclein protein (HA211157).
偶联
unconjugated
克隆号
PSH19-25
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-24] to Human beta Synuclein antibody (Capture) (HA725225) and Recombinant Human beta Synuclein protein (HA211157) as the standard. The reference range value is 156.3-10,000 pg/mL.
靶点
功能
Beta-synuclein is a protein that in humans is encoded by the SNCB gene. The protein encoded by this gene is highly homologous to alpha-synuclein. These proteins are abundantly expressed in the brain and putatively inhibit phospholipase D2 selectively. The encoded protein, which may play a role in neuronal plasticity, is abundant in neurofibrillary lesions of patients with Alzheimer's disease. This protein has been shown to be highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson's disease; however, no direct relation to Parkinson's disease has been established. Two transcript variants encoding the same protein have been found for this gene. Beta-synuclein is a synuclein protein found primarily in brain tissue and is seen mainly in presynaptic terminals. Beta-synuclein is predominantly expressed in the neocortex, hippocampus, striatum, thalamus, and cerebellum. It is not found in Lewy bodies, but it is associated with hippocampal pathology in PD and DLB. Beta-synuclein is suggested to be an inhibitor of alpha-synuclein aggregation, which occurs in neurodegenerative diseases such as Parkinson's disease. Thus, beta-synuclein may protect the central nervous system from the neurotoxic effects of alpha-synuclein and provide a novel treatment of neurodegenerative disorders.
背景文献
1. Xian M et al. beta-Synuclein Intermediates alpha-Synuclein Neurotoxicity in Parkinson\'s Disease. ACS Chem Neurosci. 2024 Jul
2. Hayashi J et al. beta-Synuclein: An Enigmatic Protein with Diverse Functionality. Biomolecules. 2022 Jan
亚细胞定位
Cytoplasm.
别名
Beta-synuclein
SNCB
图片
-
![Sandwich ELISA analysis of human beta Synuclein matched pair antibodies<br /><br />Capture: <a href="/products/HA725225" style="font-weight: bold;text-decoration: underline;">HA725225</a>, Human beta Synuclein Rabbit mAb [PSH19-24]<br />Detector: <a href="/products/HA725226" style="font-weight: bold;text-decoration: underline;">HA725226</a>, Human beta Synuclein Rabbit mAb [PSH19-25]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725225" style="font-weight: bold;text-decoration: underline;">HA725225</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human beta Synuclein protein (<a href="/products/HA211157" style="font-weight: bold;text-decoration: underline;">HA211157</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725226" style="font-weight: bold;text-decoration: underline;">HA725226</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725226_1.png?v=20260401003148)
Sandwich ELISA analysis of human beta Synuclein matched pair antibodies
Capture: HA725225, Human beta Synuclein Rabbit mAb [PSH19-24]
Detector: HA725226, Human beta Synuclein Rabbit mAb [PSH19-25]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725225) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human beta Synuclein protein (HA211157) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725226, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native SNCB in Mouse brain and Rat brain lyst samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725225" style="font-weight: bold;text-decoration: underline;">HA725225</a>, Human beta Synuclein Rabbit mAb [PSH19-24]<br />Detector: <a href="/products/HA725226" style="font-weight: bold;text-decoration: underline;">HA725226</a>, Human beta Synuclein Rabbit mAb [PSH19-25]<br /><br />The concentrations of SNCB were measured in duplicates, interpolated from the SNCB standard curve and corrected for sample dilution. Undiluted samples are Mouse brain lyst and Rat brain lyst. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNCB concentration was determined to be 444,479 pg/ml in Mouse brain lyst and 486,887 pg/ml in Rat brain lyst.](https://storage.huabio.cn/huabio/productImg/HA725226_2.png?v=20260401003148)
Interpolated concentrations of native SNCB in Mouse brain and Rat brain lyst samples based on a 1,000 µg/ml extract load.
Capture: HA725225, Human beta Synuclein Rabbit mAb [PSH19-24]
Detector: HA725226, Human beta Synuclein Rabbit mAb [PSH19-25]
The concentrations of SNCB were measured in duplicates, interpolated from the SNCB standard curve and corrected for sample dilution. Undiluted samples are Mouse brain lyst and Rat brain lyst. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNCB concentration was determined to be 444,479 pg/ml in Mouse brain lyst and 486,887 pg/ml in Rat brain lyst.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Human beta Synuclein Recombinant Rabbit Monoclonal Antibody [PSH19-24] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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