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BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

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Catalog# HA724291

BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

概述

产品名称

BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human BARD1 aa 1-450.

种属反应性

Human

验证应用

WB, IP

分子量

Predicted band size: 87 kDa

阳性对照

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa treated with 2μM MG-132 for 18 hours cell lysate.

偶联

unconjugated

克隆号

PSH22-30

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

靶点

功能

BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. Most, if not all, BRCA1 heterodimerizes with BARD1 in vivo. BARD1 and BRCA1 form a heterodimer via their N-terminal RING finger domains. The BARD1-BRCA1 interaction is observed in vivo and in vitro and is essential for BRCA1 stability. BARD1 shares homology with the two most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes, and developmentally important genes such as the polycomb group of genes. The BARD1 protein also contains three tandem ankyrin repeats.

背景文献

1. Witus SR et al. BRCA1/BARD1 is a nucleosome reader and writer. Trends Biochem Sci. 2022 Jul

2. Hawsawi YM et al. BARD1 mystery: tumor suppressors are cancer susceptibility genes. BMC Cancer. 2022 Jun

亚细胞定位

Nucleus.

UNIPROT

SwissProt: Q99728 Human

别名

BARD-1 antibody

Bard1 antibody

BARD1_HUMAN antibody

BRCA1 associated RING domain 1 antibody

BRCA1 associated RING domain gene 1 antibody

BRCA1 associated RING domain protein 1 antibody

BRCA1-associated RING domain protein 1 antibody

图片

  • Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: MDA-MB-231 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 87/100 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 87/100 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 87 kDa<br />Observed band size: 87/100 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 87 kDa
    Observed band size: 87/100 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • BARD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724291" style="font-weight: bold;text-decoration: underline;">HA724291</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    BARD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724291 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724291 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA724291 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA724291 in HeLa cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 minutes; ECL: K1801

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