VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody [PSH20-96]

Western blot analysis of VEGFR3 / FLT4 on different lysates with Rabbit anti-VEGFR3 / FLT4 antibody (HA724191) at 1/5,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: A549 treated with 10ng/mL TNF alpha and 10nM TPA for 24 hours cell lysate (20 µg/Lane)

Predicted band size: 153 kDa
Observed band size: 180 kDa

Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724191) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (HA724191) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724191) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (HA724191) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724191) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.