Mouse Periostin Recombinant Rabbit Monoclonal Antibody [PSH20-88] - BSA and Azide free (Capture)

Sandwich ELISA analysis of Mouse Periostin matched pair antibodies

Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88]
Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724183) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Periostin protein (HA211398) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724184, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Periostin in mouse serum and Neuro-2a cell culture supernatant samples.

Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88]
Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89]

The concentrations of Periostin were measured in duplicates, interpolated from the Periostin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.1% and Neuro-2a cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 6,579 ng/ml in mouse serum and undetectabel in Neuro-2a cell culture supernatant.

Interpolated concentrations of spiked Periostin in mouse cell culture media samples.

Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88]
Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89]

The concentrations of Periostin were measured in duplicates, interpolated from the Periostin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).