ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11]
Catalog# HA723427
ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11]
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WB
-
IHC-P
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Human
-
HA751440
不含抗保成分
-
unconjugated
概述
产品名称
ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ENPP1 aa 576-925.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 105 kDa
阳性对照
HepG2 cell lysate, MDA-MB-231 cell lysate, Huh7 cell lysate, human liver tissue.
偶联
unconjugated
克隆号
PSH12-11
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
-
1:1,000
靶点
功能
ENPP1 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 1) is a membrane-bound enzyme that hydrolyzes nucleotide triphosphates, such as ATP, into nucleotide monophosphates and pyrophosphate (PPi). It plays a critical role in regulating PPi levels, which are essential for inhibiting bone mineralization and soft tissue calcification. By preventing the growth of hydroxyapatite crystals, ENPP1 helps maintain proper tissue mineralization and prevents ectopic calcification. ENPP1 is involved in multiple physiological processes, including energy metabolism, purinergic signaling, and the regulation of nucleotide sugar availability in the endoplasmic reticulum and Golgi. It also modulates insulin sensitivity and function, contributing to metabolic regulation. Additionally, ENPP1 has been implicated in melanogenesis and the hydrolysis of 2',3'-cGAMP, a secondary messenger involved in immune signaling, although its role in this process remains unclear. Dysregulation or deficiency of ENPP1 is associated with various diseases, including diabetes, obesity, arterial calcification, and autoimmune disorders, making it a significant target for medical research and therapeutic development.
背景文献
1. Johnson KA, et al. Osteoblast tissue-nonspecific alkaline phosphatase antagonizes and regulates PC-1. Am J Physiol Regul Integr Comp Physiol. 2000 Oct.
2. Li L, et al. Hydrolysis of 2\'3\'-cGAMP by ENPP1 and design of nonhydrolyzable analogs. Nat Chem Biol. 2014 Dec.
亚细胞定位
Cell membrane, Basolateral cell membrane, Secreted.
UNIPROT
别名
Alkaline phosphodiesterase 1 antibody
ARHR2 antibody
COLED antibody
E-NPP 1 antibody
Ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 antibody
ENPP1 antibody
ENPP1_HUMAN antibody
Ly 41 antigen antibody
M6S1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: Huh7 cell lysate
Lane 4: THP-1 cell lysate (negative)
Lysates/proteins at 20 ug/Lane.
Predicted band size: 105 kDa
Observed band size: 140 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723427) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723427) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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