Human CXCL6/GCP-2 Recombinant Rabbit Monoclonal Antibody [PSH11-31] - BSA and Azide free (Detector)

Sandwich ELISA analysis of human CXCL6 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723314) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CTGF protein (HA211111) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723315, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL6 in human serum samples.

The concentrations of CXCL6 were measured in duplicates, interpolated from the CXCL6 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL6 concentration was determined to be 35.5 pg/ml in human serum.

Interpolated concentrations of spiked CXCL6 in human cell culture media samples.

The concentrations of CXCL6 were measured in duplicates, interpolated from the CXCL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).