FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
Catalog# HA723197
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
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WB
-
IP
-
Human
-
Mouse
-
HA751347
不含抗保成分
-
unconjugated
概述
产品名称
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human FOXM1 aa 1-400.
种属反应性
Human, Mouse
验证应用
WB, IP
分子量
Predicted band size: 84 kDa
阳性对照
HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, LO2 cell lysate, Mouse testis tissue lysate.
偶联
unconjugated
克隆号
PSH10-30
反应性数据
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
靶点
功能
Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases. FOXM1-null mouse embryos were neonatal lethal as a result of the development of polyploid cardiomyocytes and hepatocytes, highlighting the role of FOXM1 in mitotic division. More recently a study using transgenic/knockout mouse embryonic fibroblasts and human osteosarcoma cells (U2OS) has shown that FOXM1 regulates expression of a large array of G2/M-specific genes, such as Plk1, cyclin B2, Nek2 and CENPF, and plays an important role in maintenance of chromosomal segregation and genomic stability.
背景文献
1. Khan MA et al. FOXM1: A small fox that makes more tracks for cancer progression and metastasis. Semin Cancer Biol. 2023 Jul
2. Zhang Z et al. FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases. Biomolecules. 2023 May
亚细胞定位
Nucleus.
别名
FKHL16 antibody
Forkhead box M1 antibody
Forkhead box protein M1 antibody
forkhead like 16 antibody
Forkhead-related protein FKHL16 antibody
FOX M1 antibody
Foxm1 antibody
FOXM1_HUMAN antibody
FOXM1B antibody
Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
展开图片
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Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (HA723197) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: LO2 cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723197) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723197 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723197 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723197 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 4 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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