Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]
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Catalog# HA723143
Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
Liver Arginase Recombinant Rabbit Monoclonal Antibody [PSH09-78]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Liver Arginase aa 1-322/322
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 35 kDa
阳性对照
Mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.
偶联
unconjugated
克隆号
PSH09-78
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:10,000
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IHC-P
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1:2,000-1:15,000
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IF-Tissue
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1:500-1:3,000
靶点
功能
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.Defects in ARG1 are the cause of argininemia (ARGIN); also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
背景文献
1. Crombez EA, Cederbaum SD. Hyperargininemia due to liver arginase deficiency. Mol Genet Metab. 2005 Mar;84(3):243-51. doi: 10.1016/j.ymgme.2004.11.004. Epub 2004 Dec 19. PMID: 15694174.
2. Maharem TM, Zahran WE, Hassan RE, Abdel Fattah MM. Unique properties of arginase purified from camel liver cytosol. Int J Biol Macromol. 2018 Mar;108:88-97. doi: 10.1016/j.ijbiomac.2017.11.141. Epub 2017 Nov 24. PMID: 29180053.
亚细胞定位
Cytoplasm , Cytoplasmic granule
别名
A I antibody
Al antibody
ARG 1 antibody
arg1 antibody
ARGI1_HUMAN antibody
Arginase 1 antibody
Arginase liver antibody
Arginase type I antibody
Arginase, liver antibody
Arginase-1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (HA723143) at 1/10,000 dilution.
Lane 1: mouse liver tissue lysate (40 µg/Lane)
Lane 2: mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
Lane 3: rat liver tissue lysate (40 µg/Lane)
Lane 4: rat skeletal muscle tissue lysate (negative) (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 39 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723143) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/15,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Liver Arginase antibody (HA723143) at 1/15,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Liver Arginase antibody (HA723143) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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