LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20]
Catalog# HA722808
LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20]
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WB
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Human
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Mouse
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Monkey
概述
产品名称
LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human LATS1 aa 151-600.
种属反应性
Human, Mouse, Monkey
验证应用
WB
分子量
Predicted band size: 127 kDa
阳性对照
HT-29 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, C2C12 cell lysate, COS-1 cell lysate.
偶联
unconjugated
克隆号
PSH07-20
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Large tumor suppressor kinase 1 (LATS1) is an enzyme that in humans is encoded by the LATS1 gene. It has been associated with the Hippo signaling pathway, where it phosphorylates YAP and TAZ to inactivate their function. The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced histone H1 kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
背景文献
1. Xu Y et al. The N6-methyladenosine METTL3 regulates tumorigenesis and glycolysis by mediating m6A methylation of the tumor suppressor LATS1 in breast cancer. J Exp Clin Cancer Res. 2023 Jan
2. Qi S et al. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy. Mol Cell. 2022 May
亚细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body.
别名
h-warts antibody
Large tumor suppressor homolog 1 antibody
LATS large tumor suppressor homolog 1 antibody
LATS1 antibody
LATS1_HUMAN antibody
Serine threonine protein kinase LATS1 antibody
Serine/threonine-protein kinase LATS1 antibody
WARTS antibody
WARTS protein kinase antibody
wts antibody
图片
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Western blot analysis of LATS1 / WARTS on different lysates with Rabbit anti-LATS1 / WARTS antibody (HA722808) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: K-562 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: HeLa cell lysate
Lane 5: C2C12 cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722808) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"