ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]
Rmb: 880 特惠 1500
概述
产品名称
ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ATF-4 aa 1-300.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP
靶点分子量
Predicted band size: 39 kDa
阳性对照
HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 250μM CoCl2 for 6 hours cell lysate, NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate.
偶联
unconjugated
克隆号
PSH07-08
反应性数据
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
靶点
功能
Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a protein that in humans is encoded by the ATF4 gene. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix. Terminal osteoblast differentiation, represented by matrix mineralization, is significantly inhibited by the inactivation of JNK. JNK inactivation downregulates expression of ATF-4 and, subsequently, matrix mineralization. IMPACT protein regulates ATF4 in C. elegans to promote lifespan. ATF4 is also involved in the cannabinoid Δ9-tetrahydrocannabinol–induced apoptosis in cancer cells, by the proapoptotic role of the stress protein p8 via its upregulation of the endoplasmic reticulum stress-related genes ATF4, CHOP, and TRB3.
背景文献
1. He F et al. ATF4 suppresses hepatocarcinogenesis by inducing SLC7A11 (xCT) to block stress-related ferroptosis. J Hepatol. 2023 Aug
2. Xiao Y et al. Advances in the roles of ATF4 in osteoporosis. Biomed Pharmacother. 2023 Dec
亚细胞定位
Nucleus, Nucleus speckle, Cytoplasm, Cell membrane, cytoskeleton, microtubule organizing center, centrosome.
别名
Activating transcription factor 4 antibody
ATF 4 antibody
ATF4 antibody
ATF4 protein antibody
ATF4_HUMAN antibody
cAMP-dependent transcription factor ATF-4 antibody
cAMP-responsive element-binding protein 2 antibody
CREB 2 antibody
CREB-2 antibody
CREB2 antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA722796) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA722796) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 50 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
ATF4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA722796 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722796 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input)
Lane 2: HA722796 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate
Lane 3: Rabbit IgG instead of HA722796 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 40 seconds; ECL: K1802
引文
-
Reactive oxygen species-responsive hydrogel accelerates diabetic wound repair by modulating endoplasmic reticulum stress
期刊: Advanced Composites and Hybrid Materials
DOI: 10.1007/s42114-026-01842-3
IF: 15.5
应用: WB
反应种属: Mouse
发表时间: 2026 May
-
NUPR1 alleviates renal ischemia reperfusion injury by inhibiting ferroptosis involving the ATF4-CHAC1 pathway
期刊: Cellular Signalling
DOI: 10.1016/j.cellsig.2026.112547
IF: 3.7
应用: WB
反应种属: Mouse,Human
发表时间: 2026 Apr
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
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