Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH06-58]

☑ Relative expression (RE)

Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/1,000 dilution.

Lane 1: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 2: Mouse spleen tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat skeletal muscle tissue lysate (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (negative) (40 µg/Lane)

Predicted band size: 223 kDa
Observed band size: 223 kDa

Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722721) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.