CD1c Recombinant Rabbit Monoclonal Antibody [JE55-00]
Rmb: 618 特惠 1500
Catalog# HA722562
CD1c Recombinant Rabbit Monoclonal Antibody [JE55-00]
-
WB
-
IHC-P
-
mIHC
-
Human
-
unconjugated
概述
产品名称
CD1c Recombinant Rabbit Monoclonal Antibody [JE55-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD1c aa 71-120 / 333.
种属反应性
Human
验证应用
WB, IHC-P, mIHC
分子量
Predicted band size: 38 kDa
阳性对照
Jurkat cell lysates, human tonsil tissue, human spleen tissue.
偶联
unconjugated
克隆号
JE55-00
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
mIHC
-
1:100
靶点
功能
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known.
背景文献
1. Bourdely P et al. Transcriptional and Functional Analysis of CD1c(+) Human Dendritic Cells Identifies a CD163(+) Subset Priming CD8(+)CD103(+) T Cells. Immunity. 2020 Aug
2. Chen X et al. CD1C is associated with breast cancer prognosis and immune infiltrates. BMC Cancer. 2023 Feb
亚细胞定位
Cell membrane, Endosome membrane, Lysosome.
UNIPROT
别名
BDCA1 antibody
CD1 antibody
CD1A antibody
CD1c antibody
CD1c antigen antibody
CD1C antigen c polypeptide antibody
CD1c molecule antibody
CD1C_HUMAN antibody
Cortical thymocyte antigen CD1C antibody
Differentiation antigen CD1 alpha 3 antibody
展开图片
-
Western blot analysis of CD1c on Jurkat cell lysates with Rabbit anti-CD1c antibody (HA722562) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722562) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD1c antibody (HA722562) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722562) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD1c antibody (HA722562) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722562) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号